Property Description:  James Preserve, (also known as the Darwin James Preserve) is a nature preserve located  in the village of Old  Brookville in Nassau County.

The property lies in the headwaters of Cedar Swamp Creek and is made up of a freshwater marsh and swamp, oak-tulip tree forest and fields, and a trail that leads to Valentines Lane, which leads to the  Louis C. Clark Sanctuary.  The  preserve was maintained by The Nature Conservancy prior to being transferred to the Land Alliance in in 2015.  

Acres: 20 acres

James Preserve is not open to the public.